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Comparisons of the Isozymes of Arginine-Specific Proteinases in the Culture Supernatant of Porphyromonas gingivalis
https://mdu.repo.nii.ac.jp/records/741
https://mdu.repo.nii.ac.jp/records/741d99acac4-d933-413e-83de-40b2d80a0e1c
名前 / ファイル | ライセンス | アクション |
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matsumoto_shigaku_24-01-05.pdf (300.8 kB)
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Item type | Journal Article(1) | |||||
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公開日 | 2012-05-24 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Comparisons of the Isozymes of Arginine-Specific Proteinases in the Culture Supernatant of Porphyromonas gingivalis | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | P. gingivalis | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | proteinases | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | enzymology | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
その他(別言語等)のタイトル | ||||||
その他のタイトル | Porphyromonas gingivalis培養上清中のアルギニン特異的プロテアーゼのアイソザイムの比較 | |||||
著者 |
FUJIMURA, SETSUO
× FUJIMURA, SETSUO |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | By anion-exchange chromatography of the concentrated culture fluids of P. gingivalis W 50, two proteolytic enzymes, referred to as RGP-I and RGP-II were, eluted from column at the different NaCl concentrations in the eluant buffer. The two enzymes were purified until the preparations displayed single bands with sodium dodecyl sulfate-polyacryl amide gel electrophoresis (SDS-PAGE) by gel filtration and isoelectric focusing. Comparative studies between the two enzymes revealed that the same molecular masses (44 kDa) of RGP-I and II were obtained both with gel filtration and SDS-PAGE. However, the isoelectric points of RGP-I and II were 5.0 and 5.7, respectively. Both enzymes were thiol proteinases and were inhibited by sulfhydryl group blocking reagents, tosyl-L-lysine chloromethylketone, EDTA, leupeptin, L-trans-epoxy-succinylleucylamido-(4-guanidino) butane, and antipain. Both enzymes were activated by glycylglycine. RGP-I and RGP-II hydrolyzed synthetic substrates containing arginine in the P-1 positions, but those containing lysine in the same position were notcleaved. | |||||
書誌情報 |
松本歯学 巻 24, 号 1, p. 52-57, 発行日 1998-04-30 |
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出版者 | ||||||
出版者 | 松本歯科大学学会 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0385-1613 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00232590 | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |