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豚丹毒菌の迅速診断法の開発
https://az.repo.nii.ac.jp/records/5040
https://az.repo.nii.ac.jp/records/5040db829c34-87ab-4605-b5f2-137f319d0c06
名前 / ファイル | ライセンス | アクション |
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bull_jau_vol15-16-164.pdf (453.0 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2007-01-01 | |||||
タイトル | ||||||
タイトル | 豚丹毒菌の迅速診断法の開発 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Development of a rapid detection method for Erysipelothrix | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
ページ属性 | ||||||
内容記述タイプ | Other | |||||
内容記述 | P(論文) | |||||
記事種別 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 特集 | |||||
Type | ||||||
内容記述タイプ | Other | |||||
内容記述 | FEATURE ARTICLES | |||||
著者 |
岡谷, 友三アレシヤンドレ
× 岡谷, 友三アレシヤンドレ× 村上, 賢× 森田, 英利× 加藤, 行男× Okatani, Alexandre T.× Murakami, Masaru× Morita, Hidetoshi× Kato, Yukio |
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著者ID | ||||||
000336 | ||||||
著者ID | ||||||
000259 | ||||||
著者ID | ||||||
000247 | ||||||
著者ID | ||||||
000075 | ||||||
Author ID | ||||||
000336 | ||||||
Author ID | ||||||
000259 | ||||||
Author ID | ||||||
000247 | ||||||
Author ID | ||||||
000075 | ||||||
所属機関 | ||||||
麻布大学獣医学部 | ||||||
所属機関 | ||||||
麻布大学獣医学部 | ||||||
所属機関 | ||||||
麻布大学獣医学部 | ||||||
所属機関 | ||||||
麻布大学獣医学部 | ||||||
Institution or Company | ||||||
School of Veterinary Medicine, Azabu University | ||||||
Institution or Company | ||||||
School of Veterinary Medicine, Azabu University | ||||||
Institution or Company | ||||||
School of Veterinary Medicine, Azabu University | ||||||
Institution or Company | ||||||
School of Veterinary Medicine, Azabu University | ||||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | 本研究では,LAMP法を用い,gyrB遺伝子を標的とした豚丹毒菌の迅速な検出法の開発を行った。供試菌株としてE. rhusiopathiae, E. tonsillarum, Erysipelothrix spp血清型13型および血清型18型を用いた。各菌株のDNAを抽出,複数のプライマーを用いてPCRで標的遺伝子の増幅を試みた。供試したPCRプライマーのうち,プライマーUP1/UP2rでE. rhusiopathiae, E. tonsillarumおよび血清型13型で増幅産物が得られ,血清型18型の菌株からは前述のプライマーを基に設定したプライマーで増幅産物が得られた。PCRで得られた増幅産物を精製,遺伝子配列の決定を行い,LAMPプライマーを設定した。設定されたLAMPプライマーのうち,1つのプライマーセットでは供試した4菌株すべてが検出可能で,また,他のプライマーセットではE. tonsillarumおよび血清型13型の菌株の検出が可能であった。 | |||||
Abstract | ||||||
内容記述タイプ | Other | |||||
内容記述 | The aim of this study was to develop a rapid detection method of Erysipelothrix species. DNA of E. rhusiopathiae, E. tonsillarum, and Erysipelothrix spp. serovar 13 and serovar 18 were extracted and submitted for detection and sequencing of the gyrB gene. Among the primers tested for amplifying the gyrB, the universal primer set UP1UP2r showed the best result, and a DNA fragment were obtained from the E. rhusiopathiae, E. tonsillarum and Erysipelothrix spp. serovar 13. DNA amplification band from Erysipelothrix spp. serovar 18 was obtained by a primer derived from the above primer set. The obtained fragments were introduced in the pGEM-T Easy Vector and submitted for sequencing. LAMP primers sets were generated from the obtained sequence, and, among the generated primer sets, one primer set was able to detect the 4 strains tested, and another set was able to detect the E. tonsillarum and the strain of serovar 13. | |||||
書誌情報 |
麻布大学雑誌 en : Journal of Azabu University 巻 15/16, p. 164-167, 発行日 2008-03-31 |
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出版者 | ||||||
出版者 | 麻布大学 | |||||
Publisher | ||||||
出版者 | Azabu University | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1346-5880 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA11561528 | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
text version | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
format | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf |