{"_buckets": {"deposit": "e129f1e9-9d59-45e7-88d4-a6118f510985"}, "_deposit": {"created_by": 15, "id": "7", "owners": [15], "pid": {"revision_id": 0, "type": "depid", "value": "7"}, "status": "published"}, "_oai": {"id": "oai:fdc.repo.nii.ac.jp:00000007", "sets": ["6"]}, "author_link": [], "item_10006_date_granted_11": {"attribute_name": "学位授与年月日", "attribute_value_mlt": [{"subitem_dategranted": "2014-03-15"}]}, "item_10006_degree_grantor_9": {"attribute_name": "学位授与大学", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_name": "福岡歯科大学"}], "subitem_degreegrantor_identifier": [{"subitem_degreegrantor_identifier_name": "37114"}]}]}, "item_10006_degree_name_8": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士(歯学)"}]}, "item_10006_description_10": {"attribute_name": "学位授与年度", "attribute_value_mlt": [{"subitem_description": "2013年度"}]}, "item_10006_description_7": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Transforming growth factor-β1 (TGF-β1) reportedly causes the differentiation of fibroblasts to myofibroblasts during wound healing. We investigated the mechanism underlying the activation of latent TGF-β1 released by keratinocytes in efforts to identify promising pharmacological approaches for the prevention of hypertrophic scar formation. A three-dimensional collagen gel matrix culture was prepared using rat keratinocytes and dermal fibroblasts. Stratified keratinocytes promoted the TGF receptor-dependent increase in α-smooth muscle actin (α-SMA) immunostaining and mRNA levels in fibroblasts. Latent TGF-β1 was found to be localized suprabasally and secreted. α-SMA expression was inhibited by an anti-αv-integrin antibody and a matrix metalloproteinase (MMP) inhibitor, GM6001. In a two-dimensional fibroblast culture, α-SMA expression depended on the production of endogenous TGF-β1 and required αv-integrin or MMP for the response to recombinant latent TGF-β1. In keratinocyte-conditioned medium, MMP-dependent latent TGF-β1 secretion was detected. Applying this medium to the fibroblast culture enhanced α-SMA production. This effect was decreased by GM6001, the anti-αv-integrin antibody, or the preabsorption of latent TGF-β1. These results indicate that keratinocytes secrete latent TGF-β1, which is liberated to fibroblasts over distance and is activated to produce α-SMA with the aid of a positive-feedback loop. MMP inhibition was effective for targeting both keratinocytes and fibroblasts in this model.", "subitem_description_type": "Abstract"}]}, "item_10006_dissertation_number_12": {"attribute_name": "報告番号", "attribute_value_mlt": [{"subitem_dissertationnumber": "甲第253号"}]}, "item_10006_relation_14": {"attribute_name": "情報源", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "J Pharmacol Sci. 2014;124(2):230-43. Epub 2014 Jan 31."}]}]}, "item_10006_relation_15": {"attribute_name": "関連サイト", "attribute_value_mlt": [{"subitem_relation_name": [{"subitem_relation_name_text": "J-STAGE"}], "subitem_relation_type_id": {"subitem_relation_type_id_text": "https://www.jstage.jst.go.jp/article/jphs/124/2/124_13209FP/_article", "subitem_relation_type_select": "URI"}}]}, "item_10006_version_type_18": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Hata, Shozaburo"}]}, {"creatorNames": [{"creatorName": "Okamura, Kazuhiko"}]}, {"creatorNames": [{"creatorName": "Hatta, Mitsutoki"}]}, {"creatorNames": [{"creatorName": "Ishikawa, Hiroyuki"}]}, {"creatorNames": [{"creatorName": "Yamazaki, Jun"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2014-08-20"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "甲第253号_論文(秦).pdf", "filesize": [{"value": "5.9 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 5900000.0, "url": {"label": "甲第253号_論文(秦)", "objectType": "fulltext", "url": "https://fdc.repo.nii.ac.jp/record/7/files/甲第253号_論文(秦).pdf"}, "version_id": "045f8571-a878-452d-ab76-b4648faafcd4"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2014-04-28"}], "displaytype": "detail", "download_preview_message": "", "file_order": 1, "filename": "甲第253号_論文内容の要旨(秦).pdf", "filesize": [{"value": "89.7 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 89700.0, "url": {"label": "要旨", "url": "https://fdc.repo.nii.ac.jp/record/7/files/甲第253号_論文内容の要旨(秦).pdf"}, "version_id": "266d86f8-e0fa-4394-b538-ef097bf5dd16"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2014-04-28"}], "displaytype": "detail", "download_preview_message": "", "file_order": 2, "filename": "甲第253号_論文審査結果の要旨(秦).pdf", "filesize": [{"value": "297.7 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_note", "mimetype": "application/pdf", "size": 297700.0, "url": {"label": "審査結果の要旨", "url": "https://fdc.repo.nii.ac.jp/record/7/files/甲第253号_論文審査結果の要旨(秦).pdf"}, "version_id": "8462f091-5fa1-4165-ad92-64b592695bfe"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "keratinocyte", "subitem_subject_language": "en"}, {"subitem_subject": "fibroblast", "subitem_subject_language": "en"}, {"subitem_subject": "transforming growth factor-β1", "subitem_subject_language": "en"}, {"subitem_subject": "αv-integrin", "subitem_subject_language": "en"}, {"subitem_subject": "matrix metalloproteinase", "subitem_subject_language": "en"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "doctoral thesis", "resourceuri": "http://purl.org/coar/resource_type/c_db06"}]}, "item_title": "Proteolytic and non-proteolytic activation of keratinocyte-derived latent TGF-β1 induces fibroblast differentiation in a wound-healing model using rat skin.", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Proteolytic and non-proteolytic activation of keratinocyte-derived latent TGF-β1 induces fibroblast differentiation in a wound-healing model using rat skin.", "subitem_title_language": "en"}]}, "item_type_id": "10006", "owner": "15", "path": ["6"], "permalink_uri": "https://fdc.repo.nii.ac.jp/records/7", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2014-04-28"}, "publish_date": "2014-04-28", "publish_status": "0", "recid": "7", "relation": {}, "relation_version_is_last": true, "title": ["Proteolytic and non-proteolytic activation of keratinocyte-derived latent TGF-β1 induces fibroblast differentiation in a wound-healing model using rat skin."], "weko_shared_id": -1}
Proteolytic and non-proteolytic activation of keratinocyte-derived latent TGF-β1 induces fibroblast differentiation in a wound-healing model using rat skin.
https://fdc.repo.nii.ac.jp/records/7
https://fdc.repo.nii.ac.jp/records/7