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HSG cells were seeded in collagen sponges (COL-S)(three-dimensional culture) with an agitated seeding method, and cultured for various times. As a control, HSG cells were cultured on a polystyrene dish (PS) or a collagen coated dish (COL-C)(monolayer culture). The morphological features indicate that HSG cells cultured with COL-S presented a globular shape compared with monolayer culture such as with PS or COL-C. We focused on cytokeratins (CK) that are used as the marker proteins for epithelia to characterize the cultured cells. In HSG cells, CK7, CK8, CK17 and CK18 are expressed, but CK13 which is expressed specifically in mucosal epithelia is not expressed. The expression levels of CK genes were investigated by RT-PCR. The results were that the level of CK8 and CK18 were downregulated. The level of CK13 was upregulated. On the other hand, no alternation of the expression levels of CK7 and CK17 was observed. Western Blotting was carried out to investigate the expression of protein. The bands of CK8 and CK18 were detected. The expression level of CK8 and CK18 protein was reduced as well as the mRNA level. CK13 protein was not detected in this way. The expression level of the Nm23-H1 gene, which is one of tumor suppressor genes and concerned with ERK/MAPK signal transduction, was downregulated. 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コラーゲンスポンジを用いた三次元培養におけるヒト顎下腺由来腺癌細胞株の遺伝子発現
https://iwatemed.repo.nii.ac.jp/records/1337
https://iwatemed.repo.nii.ac.jp/records/1337135049ba-d39c-4cdd-ba95-f89e38af6ad9
名前 / ファイル | ライセンス | アクション |
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KJ00003984483 (1.6 MB)
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Creative Commons : 表示 - 非営利 - 改変禁止
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Item type | 紀要論文(ELS) / Departmental Bulletin Paper(1) | |||||
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公開日 | 2005-12-20 | |||||
タイトル | ||||||
タイトル | コラーゲンスポンジを用いた三次元培養におけるヒト顎下腺由来腺癌細胞株の遺伝子発現 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Gene expression in human salivary gland cells on three-dimensional culture comprised in collagen sponge | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
記事種別(日) | ||||||
研究 | ||||||
記事種別(英) | ||||||
en | ||||||
Original | ||||||
著者名(日) |
南, 順子
× 南, 順子× 加茂, 政晴× 客本, 斉子 |
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著者名よみ |
ミナミ, ジュンコ
× ミナミ, ジュンコ× カモ, マサハル× キャクモト, サイコ |
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雑誌書誌ID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00017474 | |||||
書誌情報 |
岩手医科大学歯学雑誌 en : Dental journal of Iwate Medical University 巻 30, 号 3, p. 216-227, 発行日 2005-12-20 |