WEKO3
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{"attribute_name": "著者名よみ", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "ササキ-オイカワ, アイ"}], "nameIdentifiers": [{"nameIdentifier": "8089", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "オオツ, ケイシ"}], "nameIdentifiers": [{"nameIdentifier": "8090", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "フジワラ, ナオキ"}], "nameIdentifiers": [{"nameIdentifier": "8091", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "イシゼキ, キヨト"}], "nameIdentifiers": [{"nameIdentifier": "8092", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "ナカトミ, ミツシロ"}], "nameIdentifiers": [{"nameIdentifier": "8093", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "オオシマ, ハヤト"}], "nameIdentifiers": [{"nameIdentifier": "8094", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "ハラダ, ヒデミツ"}], "nameIdentifiers": [{"nameIdentifier": "8095", "nameIdentifierScheme": 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"creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "8101", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "HARADA, Hidemitsu", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "8102", "nameIdentifierScheme": "WEKO"}]}]}, "item_2_description_1": {"attribute_name": "ページ属性", "attribute_value_mlt": [{"subitem_description": "P(論文)", "subitem_description_type": "Other"}]}, "item_2_description_11": {"attribute_name": "抄録(日)", "attribute_value_mlt": [{"subitem_description": "エナメル質の基本構造をなすエナメル小柱には横紋が観察される.この横紋は概日リズムを刻んだ成長線のひとつとして知られているが,形成メカニズムについては様々な説がある.非脱灰凍結切片で基質形成期のエナメル基質を抗アメロゲニン抗体で免疫染色すると横紋様パターンを示した.この結果から,横紋は基質形成期のアメロゲニンのタンパク量に依存した石灰化パターンであり,アメロゲニンの発現は概日的に変動するのではないかと推測した.そこで,アメロゲニンの発現に周期があるか観察するために,アメロゲニンプロモーターの下流にルシフェラーゼを繋いだコンストラクト(pGL3-1730-luc)をラットエナメル芽細胞株HAT7に遺伝子導入してアメロゲニンの転写活性を計測したところ,一定の周期をもって変動することが認められた.次に,プロモーター領域のDeletion-mutant (pGL3-464, -74, -48-luc)を作製して周期性の制御に関わる領域を検索した結果, C/EBPαのbindingモチーフが有力な候補と考えられた.その転写はMsx2によって制御を受けることが知られていることから, Msx2の発現ベクターを用いた強制発現の影響を調べたところ,そのリズムが消失した.またMsx2の遺伝子欠損マウスには基質形成の横紋様パターンが見られなかった.以上の結果から, Msx2がアメロゲニンの発現周期に影響していることが推測され,さらにアメロゲニンの発現量の周期的変化によって生じた基質形成パターンが,横紋形成に関与していると考えられた.", "subitem_description_type": "Other"}]}, "item_2_description_12": {"attribute_name": "抄録(英)", "attribute_value_mlt": [{"subitem_description": "In enamel rods, periodic bands referred to as \u0027cross-striations\u0027 are observed, and known as incremental lines of circadian rhythm. Though it is considered that the cross-striation is involved in the biological circadian rhythm during the secretion of enamel matrix protein by ameloblasts, the developmental mechanism involved has not been examined in detail. By immunostaining amelogenin in fresh frozen sections of mouse lower incisor, we could observe fluorescent periodic bands in the enamel matrix, which were identical to the pattern of these cross-striations. Accordingly, we focused on the biological rhythm in the section of amelogenin. We examined amelogenin mRNA transcriptional activity in an ameloblast cell line (HAT7) by using real-time luminescence microscopy. The results showed that amelogenin mRNA transcriptional activity exhibited periodic rhythmicity and that Msx2 over-expression led to the disappearance of the periodic change. Further, in the lower incisors of Msx2-deficient mice, the periodic bands were not observed. Taken together, our findings suggest that the formation of cross-striations in the enamel rods was associated with the expression of amelogenin regulated by the transcriptional activity.", "subitem_description_type": "Other"}]}, "item_2_source_id_13": {"attribute_name": "雑誌書誌ID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00017474", "subitem_source_identifier_type": "NCID"}]}, "item_2_text_10": {"attribute_name": "著者所属(英)", "attribute_value_mlt": [{"subitem_text_language": "en", "subitem_text_value": "Division of Developmental Biology and Regenerative Medicine, Department of Anatomy, Iwate Medical University"}, {"subitem_text_language": "en", "subitem_text_value": "Advanced Oral Health Science Research Center, Iwate Medical University"}, {"subitem_text_language": "en", "subitem_text_value": "Division of Developmental Biology and Regenerative Medicine, Department of Anatomy, Iwate Medical University"}, {"subitem_text_language": "en", "subitem_text_value": "Division of Developmental Biology and Regenerative Medicine, Department of Anatomy, Iwate Medical University"}, {"subitem_text_language": "en", "subitem_text_value": "Division of Anatomy and Cell Biology of the Hard Tissue, Department of Tissue Regeneration and Reconstruction, Niigata University Graduate School of Medical and Dental Sciences"}, {"subitem_text_language": "en", "subitem_text_value": "Division of Anatomy and Cell Biology of the Hard Tissue, Department of Tissue Regeneration and Reconstruction, Niigata University Graduate School of Medical and Dental Sciences"}, {"subitem_text_language": "en", "subitem_text_value": "Division of Developmental Biology and Regenerative Medicine, Department of Anatomy, Iwate Medical University"}]}, "item_2_text_9": {"attribute_name": "著者所属(日)", "attribute_value_mlt": [{"subitem_text_value": "岩手医科大学解剖学講座発生生物・再生医学分野"}, {"subitem_text_value": "岩手医科大学先進歯科医療研究センター"}, {"subitem_text_value": "岩手医科大学解剖学講座発生生物・再生医学分野"}, {"subitem_text_value": "岩手医科大学解剖学講座発生生物・再生医学分野"}, {"subitem_text_value": "新潟大学大学院医歯学総合研究科顎顔面再建学講座硬組織形態学分野"}, {"subitem_text_value": "新潟大学大学院医歯学総合研究科顎顔面再建学講座硬組織形態学分野"}, {"subitem_text_value": "岩手医科大学解剖学講座発生生物・再生医学分野"}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2012-05-11"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "KJ00008045758.pdf", "filesize": [{"value": "15.0 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensefree": "Creative Commons : 表示 - 非営利 - 改変禁止", "licensetype": "license_free", "mimetype": "application/pdf", "size": 15000000.0, "url": {"label": "KJ00008045758", "url": "https://iwatemed.repo.nii.ac.jp/record/2395/files/KJ00008045758.pdf"}, "version_id": "fba50508-d71e-44a7-9362-ff1eb067649c"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "enamel", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "amelogenin", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "cross-striation", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "circadian rhythm", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "エナメル質の横紋形成メカニズムの解明", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "エナメル質の横紋形成メカニズムの解明"}, {"subitem_title": "Elucidation of enamel cross-striation formation mechanism", "subitem_title_language": "en"}]}, "item_type_id": "2", "owner": "5", "path": ["125", "560", "574", "577"], "permalink_uri": "https://iwatemed.repo.nii.ac.jp/records/2395", "pubdate": {"attribute_name": "公開日", "attribute_value": "2012-05-11"}, "publish_date": "2012-05-11", "publish_status": "0", "recid": "2395", "relation": {}, "relation_version_is_last": true, "title": ["エナメル質の横紋形成メカニズムの解明"], "weko_shared_id": 5}
エナメル質の横紋形成メカニズムの解明
https://iwatemed.repo.nii.ac.jp/records/2395
https://iwatemed.repo.nii.ac.jp/records/2395b3b4bb00-c4a0-481f-908a-396d5ba615ae
名前 / ファイル | ライセンス | アクション |
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KJ00008045758 (15.0 MB)
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Creative Commons : 表示 - 非営利 - 改変禁止
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Item type | 紀要論文(ELS) / Departmental Bulletin Paper(1) | |||||
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公開日 | 2012-05-11 | |||||
タイトル | ||||||
タイトル | エナメル質の横紋形成メカニズムの解明 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Elucidation of enamel cross-striation formation mechanism | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者名(日) |
及川, 愛
× 及川, 愛× 大津, 圭史× 藤原, 尚樹× 石関, 清人× 中富, 満城× 大島, 勇人× 原田, 英光 |
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著者名よみ |
ササキ-オイカワ, アイ
× ササキ-オイカワ, アイ× オオツ, ケイシ× フジワラ, ナオキ× イシゼキ, キヨト× ナカトミ, ミツシロ× オオシマ, ハヤト× ハラダ, ヒデミツ |
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雑誌書誌ID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00017474 | |||||
書誌情報 |
岩手医科大学歯学雑誌 en : Dental journal of Iwate Medical University 巻 37, 号 1, p. 14-23, 発行日 2012-05-11 |