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Characterisation of an intrinsically disordered protein complex of Swi5-Sfr1 by ion mobility mass spectrometry and small-angle X-ray scattering.
https://doi.org/10.15015/00001559
https://doi.org/10.15015/0000155940a1cbd3-03ee-4ce2-85c3-7e1ecf8a3f56
名前 / ファイル | ライセンス | アクション |
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本文 (623.6 kB)
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Copyright©2019 by Yokohama City University
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2019-02-06 | |||||
タイトル | ||||||
タイトル | Characterisation of an intrinsically disordered protein complex of Swi5-Sfr1 by ion mobility mass spectrometry and small-angle X-ray scattering. | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Characterisation of an intrinsically disordered protein complex of Swi5-Sfr1 by ion mobility mass spectrometry and small-angle X-ray scattering. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Amino Acid Sequence | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Cell Cycle Proteins | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Models, Molecular | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Molecular Sequence Data | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Saccharomyces cerevisiae Proteins | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Scattering, Small Angle | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Schizosaccharomyces | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Schizosaccharomyces pombe Proteins | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Spectrometry, Mass, Electrospray Ionization | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Transcription Factors | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | X-Ray Diffraction | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
ID登録 | ||||||
ID登録 | 10.15015/00001559 | |||||
ID登録タイプ | JaLC | |||||
著者 |
明石, 知子
× 明石, 知子× Saikusa, Kazumi× Kuwabara, Naoyuki× Kokabu, Yuichi× Inoue, Yu× Sato, Mamoru× Iwasaki, Hiroshi× Shimizu, Toshiyuki× Ikeguchi, Mitsunori× Akashi, Satoko |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | It is now recognized that intrinsically disordered proteins (IDPs) play important roles as hubs in intracellular networks, and their structural characterisation is of significance. However, due to their highly dynamic features, it is challenging to investigate the structures of IDPs solely by conventional methods. In the present study, we demonstrate a novel method to characterise protein complexes using electrospray ionization ion mobility mass spectrometry (ESI-IM-MS) in combination with small-angle X-ray scattering (SAXS). This method enables structural characterisation even of proteins that have difficulties in crystallisation. With this method, we have characterised the Schizosaccharomyces pombe Swi5-Sfr1 complex, which is expected to have a long disordered region at the N-terminal portion of Sfr1. ESI-IM-MS analysis of the Swi5-Sfr1 complex revealed that its experimental collision cross-section (CCS) had a wide distribution, and the CCS values of the most dominant ions were ∼56% of the theoretically calculated value based on the SAXS low-resolution model, suggesting a significant size reduction in the gas phase. The present study demonstrates that the newly developed method for calculation of the theoretical CCSs of the SAXS low-resolution models of proteins allows accurate evaluation of the experimental CCS values of IDPs provided by ESI-IM-MS by comparing with the low-resolution solution structures. Furthermore, it was revealed that the combination of ESI-IM-MS and SAXS is a promising method for structural characterisation of protein complexes that are unable to crystallise. | |||||
書誌情報 |
en : The Analyst 巻 138, 号 5, p. 1441-1449, 発行日 2013-03-07 |
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ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 1364-5528 | |||||
PubMed番号 | ||||||
識別子タイプ | PMID | |||||
関連識別子 | 23324799 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1039/c2an35878f |