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Successful cryopreservation of spermatogonia in critically endangered Manchurian trout (Brachymystax lenok)
https://oacis.repo.nii.ac.jp/records/1631
https://oacis.repo.nii.ac.jp/records/163127e3078a-1ca3-48a4-a183-2acd6758efa5
名前 / ファイル | ライセンス | アクション |
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25114005_cryobiology_72-2-165 (639.5 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2019-01-07 | |||||
タイトル | ||||||
タイトル | Successful cryopreservation of spermatogonia in critically endangered Manchurian trout (Brachymystax lenok) | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題 | Manchurian trout, Cryopreservation, Slow freezing, Spermatogonial transplantation, Spermatogonial stem cells | |||||
資源タイプ | ||||||
資源タイプ | journal article | |||||
著者 |
Lee, Seungki
× Lee, Seungki× Yoshizaki, Goro |
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書誌情報 |
Cryobiology 巻 72, 号 2, p. 165-168, 発行日 2016-04 |
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抄録 | ||||||
内容記述 | Because of the lack of cryopreservation techniques for fish eggs and embryos, 22 cryopreservation of fish spermatogonia and subsequent generation of eggs and sperm would be 23 an exit strategy for the long-term preservation of genetic resources. This study aimed to optimize 24 cryoprotectants, cooling rates, and thawing temperatures for slow freezing of spermatogonia from 25 endangered Manchurian trout (Brachymystax lenok). Whole testes were frozen with a 26 cryomedium containing 1.3 M methanol, 0.2 M trehalose, and 10% egg yolk at a cooling rate of 27 −1°C/min and then stored in liquid nitrogen for 2 days. After thawing at 30°C in a water bath, 28 testicular cells from thawed testes were intraperitoneally transplanted into allogeneic triploid 29 hatchlings. Transplanted spermatogonia migrated toward and were incorporated into recipient 30 gonads, where they underwent gametogenesis. Transplantation efficiency did not significantly 31 differ between frozen and fresh testes, demonstrating that Manchurian trout spermatogonia can be 32 successfully cryopreserved in liquid nitrogen. | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00112240 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00620601 | |||||
PubMed番号 | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | PMID | |||||
関連識別子 | 26827783 | |||||
DOI | ||||||
関連タイプ | isVersionOf | |||||
識別子タイプ | DOI | |||||
関連識別子 | 10.1016/j.cryobiol.2016.01.004 | |||||
権利 | ||||||
権利情報 | (c) 2016 Elsevier Inc.. This is the author's version of the work. It is posted here for your personal use. Not for redistribution. The definitive Version of Record was published in https://doi.org/10.1016/j.cryobiol.2016.01.004 | |||||
情報源 | ||||||
関連識別子 | https://doi.org/10.1016/j.cryobiol.2016.01.004 | |||||
関連名称 | Publisher's Version/PDF | |||||
情報源 | ||||||
関連識別子 | https://kaken.nii.ac.jp/ja/grant/KAKENHI-PLANNED-25114005/ | |||||
関連名称 | KAKEN研究課題: サケ科魚類の進化に伴うGSC制御機構の変化 | |||||
情報源 | ||||||
関連識別子 | https://nrid.nii.ac.jp/ja/nrid/1000070281003/ | |||||
関連名称 | 研究代表者: 吉崎 悟朗 (東京海洋大学) | |||||
著者版フラグ | ||||||
出版タイプ | AM | |||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||
件名 | ||||||
主題 | 科学研究費研究成果 | |||||
出版者 | ||||||
出版者 | Elsevier | |||||
科学研究費研究課題 | ||||||
主題 | サケ科魚類の進化に伴うGSC制御機構の変化 | |||||
科学研究費研究課題 | ||||||
主題 | Evolution of GSC regulation in salmonids Planned | |||||
研究課題番号 | ||||||
内容記述 | 25114005 |