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ドコサヘキサエン酸はSTAT3 のリン酸化を抑制し、腎癌細胞の増殖と浸潤を抑制する
https://saitama-med.repo.nii.ac.jp/records/622
https://saitama-med.repo.nii.ac.jp/records/622ac123702-5be4-43bf-8c32-d9be0ee8e3fe
名前 / ファイル | ライセンス | アクション |
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論文内容の要旨 (124.1 kB)
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論文審査の結果の要旨 (125.7 kB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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公開日 | 2018-05-30 | |||||
タイトル | ||||||
タイトル | ドコサヘキサエン酸はSTAT3 のリン酸化を抑制し、腎癌細胞の増殖と浸潤を抑制する | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Docosahexaenoic acid inhibits the phosphorylation of STAT3 and the growth and invasion of renal cancer cells. | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | anti-tumor effect | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | docosahexaenoic acid | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | epidermal growth factor receptor | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | kidney cancer | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | signal transducer and activator of transcription 3 | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_46ec | |||||
資源タイプ | thesis | |||||
著者 |
田崎, 新資
× 田崎, 新資× Tasaki, Shinsuke |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Docosahexaenoic acid (DHA) has a variety of anti-tumor activities. The present study examined the anti-tumor activity of DHA in renal cancer cells and its underlying mechanisms of action. The effects of DHA on the viability and proliferation of the human renal cancer cell lines Caki-1 and 786-O were examined by an MTS assay and cell counting. In addition, cell cycle distribution and cell apoptosis were analyzed by flow cytometry and Annexin V staining, and modulation of cell mobility and invasiveness was assessed by wound healing and Matrigel invasion assays. Effects of DHA on intracellular signaling pathways were also analyzed by western blotting. It was observed that DHA significantly reduced the viability and proliferation of Caki-1 and 786-O cells (P<0.01). Specifically, there were increases in the sub-G1 and G2/M cell populations, as well as the percentages of cells exhibiting Annexin-positive and propidium-iodide-negative staining. In addition, the covered area in a wound and the number of cells invading through a Matrigel chamber decreased when Caki-1 or 786-O cells were treated with DHA. Phosphorylation of epidermal growth factor receptor was also upregulated following DHA treatment, while phosphorylation of signal transducer and activator of transcription 3 and Akt was downregulated. Collectively, these data suggest that DHA may be useful in the treatment of renal cell carcinoma. | |||||
学位名 | ||||||
学位名 | 博士(医学) | |||||
学位授与機関 | ||||||
学位授与機関名 | 埼玉医科大学 | |||||
学位授与年度 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 平成29年度 | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2018-01-26 | |||||
学位授与番号 | ||||||
学位授与番号 | 32409乙第1370号 | |||||
掲載誌名 | ||||||
関連名称 | Experimental and Therapeutic Medicine | |||||
掲載誌情報 | ||||||
識別子タイプ | URI | |||||
関連識別子 | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5526154/ | |||||
関連名称 | 出版社サイト |