WEKO3
アイテム
{"_buckets": {"deposit": "5a3a1fcb-964d-4d96-9692-30eac3bcbe61"}, "_deposit": {"created_by": 14, "id": "23", "owners": [14], "pid": {"revision_id": 0, "type": "depid", "value": "23"}, "status": "published"}, "_oai": {"id": "oai:osaka-dent.repo.nii.ac.jp:00000023", "sets": ["8"]}, "author_link": ["421", "56"], "item_10006_alternative_title_1": {"attribute_name": "その他(別言語等)のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "アテロコラーゲンスポンジを用いたヒト頬脂肪体由来の脱分化脂肪細胞と脂肪幹細胞の軟骨分化"}]}, "item_10006_date_granted_11": {"attribute_name": "学位授与年月日", "attribute_value_mlt": [{"subitem_dategranted": "2015-03-06"}]}, "item_10006_degree_grantor_9": {"attribute_name": "学位授与機関", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_name": "大阪歯科大学"}], "subitem_degreegrantor_identifier": [{"subitem_degreegrantor_identifier_name": "34408", "subitem_degreegrantor_identifier_scheme": "kakenhi"}]}]}, "item_10006_degree_name_8": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士(歯学)"}]}, "item_10006_description_10": {"attribute_name": "学位授与年度", "attribute_value_mlt": [{"subitem_description": "2014年度", "subitem_description_type": "Other"}]}, "item_10006_description_7": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "We evaluated the chondrocyte differentiation potential of dedifferentiated fat cells (DFATs) and adipose stem cells (ASCs) from the human buccal fat pad (BFP). We isolated the cells from two patients who underwent oral and maxillofacial surgery. Chondrocyte differentiation was evaluated based on gene and protein expression analysis in addition to histological analysis of DFATs and ASCs seeded in an atelocollagen sponge and cultured for up to 21 days. The gene expression levels of chondrocyte differentiation markers such as aggrecan, collagen type 2, and SOX9 were higher in DFATs than in ASCs cultured for 14 and 21 days, whereas protein expression levels were higher in DFATs at all time points tested. Additionally, the levels of the embryonic stem cell markers Nanog, SOX2, and OCT4 were higher in DFATs than in ASCs at 72 h. The extracellular matrix of both the cultured ASCs and DFATs was Alcian blue-positive, indicating production of sulfated glycosaminoglycans, and was aggrecan-positive. The chondrocyte differentiation ability of human DFATs was higher than that of ASCs. Isolation of DFATs from the BFP offers an aesthetic advantage, as the BFP can be obtained from the oral cavity without surface scarring. Therefore, we propose that BFP-derived DFATs are an ideal cell source for cartilage tissue engineering. This study provides evidence that DFATs from the BFP are an ideal cell source for cartilage tissue engineering.", "subitem_description_type": "Abstract"}]}, "item_10006_dissertation_number_12": {"attribute_name": "学位授与番号", "attribute_value_mlt": [{"subitem_dissertationnumber": "甲第763号"}]}, "item_10006_publisher_5": {"attribute_name": "公開者", "attribute_value_mlt": [{"subitem_publisher": "大阪歯科学会"}]}, "item_10006_version_type_18": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "西尾, 謙宏"}, {"creatorName": "ニシオ, アキヒロ", "creatorNameLang": "ja-Kana"}], "nameIdentifiers": [{"nameIdentifier": "421", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nishio, Akihiro", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "56", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-08-01"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "kou_763_txt..PDF", "filesize": [{"value": "900.1 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 900100.0, "url": {"label": "学位論文", "url": "https://osaka-dent.repo.nii.ac.jp/record/23/files/kou_763_txt..PDF"}, "version_id": "295cda45-8884-43d0-933f-3b2e03967555"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-08-01"}], "displaytype": "detail", "download_preview_message": "", "file_order": 1, "filename": "kou_763.pdf", "filesize": [{"value": "221.2 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 221200.0, "url": {"label": "論文内容要旨・審査結果要旨", "url": "https://osaka-dent.repo.nii.ac.jp/record/23/files/kou_763.pdf"}, "version_id": "9ab298d6-745d-4190-9aa0-510175170ec1"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Dedifferentiated fat cells", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Adipose stem cells", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Ceiling culture", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Buccal fat pad", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Chondrocyte differentiation", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Cartilage tissue engineering", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Dedifferentiated fat cells", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Adipose stem cells", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Ceiling culture", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Buccal fat pad", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Chondrocyte differentiation", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Cartilage tissue engineering", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "doctoral thesis", "resourceuri": "http://purl.org/coar/resource_type/c_db06"}]}, "item_title": "Chondrocyte differentiation of human buccal fat pad-derived dedifferentiated fat cells and adipose stem cells using an atelocollagen sponge", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Chondrocyte differentiation of human buccal fat pad-derived dedifferentiated fat cells and adipose stem cells using an atelocollagen sponge"}]}, "item_type_id": "10006", "owner": "14", "path": ["8"], "permalink_uri": "https://osaka-dent.repo.nii.ac.jp/records/23", "pubdate": {"attribute_name": "公開日", "attribute_value": "2016-08-01"}, "publish_date": "2016-08-01", "publish_status": "0", "recid": "23", "relation": {}, "relation_version_is_last": true, "title": ["Chondrocyte differentiation of human buccal fat pad-derived dedifferentiated fat cells and adipose stem cells using an atelocollagen sponge"], "weko_shared_id": 14}
Chondrocyte differentiation of human buccal fat pad-derived dedifferentiated fat cells and adipose stem cells using an atelocollagen sponge
https://osaka-dent.repo.nii.ac.jp/records/23
https://osaka-dent.repo.nii.ac.jp/records/23598ee2df-2c4d-45c7-8b9d-c52583bac726
名前 / ファイル | ライセンス | アクション |
---|---|---|
学位論文 (900.1 kB)
|
||
論文内容要旨・審査結果要旨 (221.2 kB)
|
Item type | 学位論文 / Thesis or Dissertation(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2016-08-01 | |||||
本文言語 | ||||||
言語 | eng | |||||
タイトル | ||||||
タイトル | Chondrocyte differentiation of human buccal fat pad-derived dedifferentiated fat cells and adipose stem cells using an atelocollagen sponge | |||||
著者 |
西尾, 謙宏
× 西尾, 謙宏× Nishio, Akihiro |
|||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Dedifferentiated fat cells | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Adipose stem cells | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Ceiling culture | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Buccal fat pad | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Chondrocyte differentiation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Cartilage tissue engineering | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Dedifferentiated fat cells | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Adipose stem cells | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Ceiling culture | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Buccal fat pad | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Chondrocyte differentiation | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Cartilage tissue engineering | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
資源タイプ | doctoral thesis | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | We evaluated the chondrocyte differentiation potential of dedifferentiated fat cells (DFATs) and adipose stem cells (ASCs) from the human buccal fat pad (BFP). We isolated the cells from two patients who underwent oral and maxillofacial surgery. Chondrocyte differentiation was evaluated based on gene and protein expression analysis in addition to histological analysis of DFATs and ASCs seeded in an atelocollagen sponge and cultured for up to 21 days. The gene expression levels of chondrocyte differentiation markers such as aggrecan, collagen type 2, and SOX9 were higher in DFATs than in ASCs cultured for 14 and 21 days, whereas protein expression levels were higher in DFATs at all time points tested. Additionally, the levels of the embryonic stem cell markers Nanog, SOX2, and OCT4 were higher in DFATs than in ASCs at 72 h. The extracellular matrix of both the cultured ASCs and DFATs was Alcian blue-positive, indicating production of sulfated glycosaminoglycans, and was aggrecan-positive. The chondrocyte differentiation ability of human DFATs was higher than that of ASCs. Isolation of DFATs from the BFP offers an aesthetic advantage, as the BFP can be obtained from the oral cavity without surface scarring. Therefore, we propose that BFP-derived DFATs are an ideal cell source for cartilage tissue engineering. This study provides evidence that DFATs from the BFP are an ideal cell source for cartilage tissue engineering. | |||||
学位名 | ||||||
学位名 | 博士(歯学) | |||||
学位授与機関 | ||||||
学位授与機関識別子Scheme | kakenhi | |||||
学位授与機関識別子 | 34408 | |||||
学位授与機関名 | 大阪歯科大学 | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2015-03-06 | |||||
学位授与番号 | ||||||
学位授与番号 | 甲第763号 |