WEKO3
アイテム
{"_buckets": {"deposit": "26a08cd1-97ec-4114-99a1-5aa818d0e31b"}, "_deposit": {"created_by": 14, "id": "28", "owners": [14], "pid": {"revision_id": 0, "type": "depid", "value": "28"}, "status": "published"}, "_oai": {"id": "oai:osaka-dent.repo.nii.ac.jp:00000028", "sets": ["9"]}, "author_link": ["394", "74"], "item_10006_alternative_title_1": {"attribute_name": "その他(別言語等)のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "IL-17A は JNK リン酸化と c-Fos 発現を抑えることにより RANKL 刺激されたRAW264.7 細胞の破⾻細胞分化を抑制する"}]}, "item_10006_date_granted_11": {"attribute_name": "学位授与年月日", "attribute_value_mlt": [{"subitem_dategranted": "2014-12-24"}]}, "item_10006_degree_grantor_9": {"attribute_name": "学位授与機関", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_name": "大阪歯科大学"}], "subitem_degreegrantor_identifier": [{"subitem_degreegrantor_identifier_name": "34408", "subitem_degreegrantor_identifier_scheme": "kakenhi"}]}]}, "item_10006_degree_name_8": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士(歯学)"}]}, "item_10006_description_10": {"attribute_name": "学位授与年度", "attribute_value_mlt": [{"subitem_description": "2014年度", "subitem_description_type": "Other"}]}, "item_10006_description_7": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "Periodontitis is a chronic inflammatory disease characterized by alveolar bone resorption. Inflammation-mediated bone loss is a major cause of various bone diseases, such as chronic periodontitis, and is due to an imbalance in bone remodeling that favors resorption. This imbalance is caused by increased inflammatory cytokines. Interleukin-17A (IL-17A) is a proinflammatory cytokine that is mainly secreted by activated T cells. IL-17A stimulates osteoclastic bone resorption via osteoblasts by inducing the expression of the receptor activator of NF-kB ligand (RANKL). However, little is known about the direct effects of IL-17A on the osteoclast precursors. We confirmed that IL-17A suppresses the osteoclast differentiation of RAW264.7 cells in the presence of RANKL in a dose-dependent manner. We also found that treatment with SP 600125, a specific inhibitor of c-Jun N-terminal kinase (JNK), significantly inhibits the TRAP activity of RAW264.7 cells, which were stimulated by RANKL. In addition, we found that IL-17 A reduces the phosphorylation of JNK and expressions of c-Fos, which were increased by RANKL stimulation. These results suggest that IL-17A-induces inhibition of JNK phosphorylation and that expression of c-Fos may be one of the factors that suppresses the differentiation of osteoclast precursors into osteoclasts.", "subitem_description_type": "Abstract"}]}, "item_10006_dissertation_number_12": {"attribute_name": "学位授与番号", "attribute_value_mlt": [{"subitem_dissertationnumber": "乙第1590号"}]}, "item_10006_publisher_5": {"attribute_name": "公開者", "attribute_value_mlt": [{"subitem_publisher": "大阪歯科学会"}]}, "item_10006_version_type_18": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_access_right": {"attribute_name": "アクセス権", "attribute_value_mlt": [{"subitem_access_right": "open access", "subitem_access_right_uri": "http://purl.org/coar/access_right/c_abf2"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "野阪, 興"}, {"creatorName": "ノサカ, コウ", "creatorNameLang": "ja-Kana"}], "nameIdentifiers": [{"nameIdentifier": "394", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nosaka, Ko", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "74", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-08-01"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "otu_1590.txt.pdf", "filesize": [{"value": "154.7 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 154700.0, "url": {"label": "学位論文", "url": "https://osaka-dent.repo.nii.ac.jp/record/28/files/otu_1590.txt.pdf"}, "version_id": "250ab5a8-f0c4-45b1-ae44-2245eeabcaa2"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2016-08-01"}], "displaytype": "detail", "download_preview_message": "", "file_order": 1, "filename": "otu_1590.pdf", "filesize": [{"value": "186.9 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_11", "mimetype": "application/pdf", "size": 186900.0, "url": {"label": "論文内容要旨・審査結果要旨", "url": "https://osaka-dent.repo.nii.ac.jp/record/28/files/otu_1590.pdf"}, "version_id": "a48db299-ea7c-4411-b17f-6406a46fa42e"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Interleukin-17A (IL-17A)", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Osteoclast differentiation", "subitem_subject_scheme": "Other"}, {"subitem_subject": "RAW264.7 cells", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Interleukin-17A (IL-17A)", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Osteoclast differentiation", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}, {"subitem_subject": "RAW264.7 cells", "subitem_subject_language": "en", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "doctoral thesis", "resourceuri": "http://purl.org/coar/resource_type/c_db06"}]}, "item_title": "IL-17A inhibits osteoclast differentiation of RANKL-stimulated RAW264.7 cells by suppressing JNK phosphorylation and c-Fos expression", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "IL-17A inhibits osteoclast differentiation of RANKL-stimulated RAW264.7 cells by suppressing JNK phosphorylation and c-Fos expression"}]}, "item_type_id": "10006", "owner": "14", "path": ["9"], "permalink_uri": "https://osaka-dent.repo.nii.ac.jp/records/28", "pubdate": {"attribute_name": "公開日", "attribute_value": "2016-08-01"}, "publish_date": "2016-08-01", "publish_status": "0", "recid": "28", "relation": {}, "relation_version_is_last": true, "title": ["IL-17A inhibits osteoclast differentiation of RANKL-stimulated RAW264.7 cells by suppressing JNK phosphorylation and c-Fos expression"], "weko_shared_id": 14}
IL-17A inhibits osteoclast differentiation of RANKL-stimulated RAW264.7 cells by suppressing JNK phosphorylation and c-Fos expression
https://osaka-dent.repo.nii.ac.jp/records/28
https://osaka-dent.repo.nii.ac.jp/records/28176c67ff-e74b-4c84-aa43-5cf2ed702b45
名前 / ファイル | ライセンス | アクション |
---|---|---|
学位論文 (154.7 kB)
|
||
論文内容要旨・審査結果要旨 (186.9 kB)
|
Item type | 学位論文 / Thesis or Dissertation(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2016-08-01 | |||||
本文言語 | ||||||
言語 | eng | |||||
タイトル | ||||||
タイトル | IL-17A inhibits osteoclast differentiation of RANKL-stimulated RAW264.7 cells by suppressing JNK phosphorylation and c-Fos expression | |||||
著者 |
野阪, 興
× 野阪, 興× Nosaka, Ko |
|||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Interleukin-17A (IL-17A) | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Osteoclast differentiation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | RAW264.7 cells | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Interleukin-17A (IL-17A) | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Osteoclast differentiation | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | RAW264.7 cells | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
資源タイプ | doctoral thesis | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Periodontitis is a chronic inflammatory disease characterized by alveolar bone resorption. Inflammation-mediated bone loss is a major cause of various bone diseases, such as chronic periodontitis, and is due to an imbalance in bone remodeling that favors resorption. This imbalance is caused by increased inflammatory cytokines. Interleukin-17A (IL-17A) is a proinflammatory cytokine that is mainly secreted by activated T cells. IL-17A stimulates osteoclastic bone resorption via osteoblasts by inducing the expression of the receptor activator of NF-kB ligand (RANKL). However, little is known about the direct effects of IL-17A on the osteoclast precursors. We confirmed that IL-17A suppresses the osteoclast differentiation of RAW264.7 cells in the presence of RANKL in a dose-dependent manner. We also found that treatment with SP 600125, a specific inhibitor of c-Jun N-terminal kinase (JNK), significantly inhibits the TRAP activity of RAW264.7 cells, which were stimulated by RANKL. In addition, we found that IL-17 A reduces the phosphorylation of JNK and expressions of c-Fos, which were increased by RANKL stimulation. These results suggest that IL-17A-induces inhibition of JNK phosphorylation and that expression of c-Fos may be one of the factors that suppresses the differentiation of osteoclast precursors into osteoclasts. | |||||
学位名 | ||||||
学位名 | 博士(歯学) | |||||
学位授与機関 | ||||||
学位授与機関識別子Scheme | kakenhi | |||||
学位授与機関識別子 | 34408 | |||||
学位授与機関名 | 大阪歯科大学 | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2014-12-24 | |||||
学位授与番号 | ||||||
学位授与番号 | 乙第1590号 |