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Combination of necroptosis and apoptosis inhibition enhances cardioprotection against myocardial ischemia-reperfusion injury
https://osaka-dent.repo.nii.ac.jp/records/43
https://osaka-dent.repo.nii.ac.jp/records/43d3c168ad-1ad7-419d-a0bc-16f82f1a4218
名前 / ファイル | ライセンス | アクション |
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学位論文 (442.3 kB)
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論文内容要旨・審査結果要旨 (127.2 kB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
---|---|---|---|---|---|---|
公開日 | 2016-08-01 | |||||
本文言語 | ||||||
言語 | eng | |||||
タイトル | ||||||
タイトル | Combination of necroptosis and apoptosis inhibition enhances cardioprotection against myocardial ischemia-reperfusion injury | |||||
著者 |
越沼, 静
× 越沼, 静× Koshinuma, Shizuka |
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キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Amino Acid Chloromethyl Ketones | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Animals | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Apoptosis | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Cardiotonic Agents | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Caspase 3 | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Drug Therapy, Combination | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Guinea Pigs | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Heart | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Hemodynamics | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Imidazoles | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Indoles | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Male | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Myocardial Reperfusion Injury | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Myocardium | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Necrosis | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
資源タイプ | doctoral thesis | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Necroptosis has been proposed as a mode of cell death that is a caspase-independent programmed necrosis. We investigated whether necroptosis is involved in myocardial ischemia-reperfusion injury in isolated guinea pig hearts and, if so, whether simultaneous inhibition of necroptosis and apoptosis confers enhanced cardioprotection. | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Isolated perfused guinea pig hearts were subjected to 30 min ischemia and 4 h reperfusion (control = CTL, n = 8). Necrostatin-1 (necroptosis inhibitor, 10 μM), Z-VAD (apoptosis inhibitor, 0.1 μM) and both inhibitors were administered starting 5 min before ischemia and during the initial 30 min of reperfusion (Nec, Z-VAD, Nec + Z-VAD; n = 8 each). Contractile recovery was monitored by left ventricular developed (LVDP) and end-diastolic (LVEDP) pressure. Infarct size was determined by triphenyltetrazolium chloride staining. Tissue samples were obtained after 4 h reperfusion to determine expression of receptor-interacting protein 1 (RIP1) and activated caspase 3 by Western blot analysis. | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | After reperfusion, Nec + Z-VAD had higher LVDP and lower LVEDP compared with CTL. Infarct size was reduced in Nec and Z-VAD compared with CTL. Combination of necroptosis and apoptosis inhibition further reduced infarct size. Expression of activated caspase 3 was not increased in Z-VAD and Nec + Z-VAD compared with Nec and CTL. Expression of RIP1 was preserved in Z-VAD and Nec + Z-VAD compared with CTL, suggesting RIP1-mediated necrosis is involved in myocardial ischemia-reperfusion injury. | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Necroptosis is involved in myocardial ischemia-reperfusion injury, and simultaneous inhibition of necroptosis and apoptosis enhances the cardioprotective effect. These findings may provide a novel, additive strategy for cardioprotection in acute myocardial infarction. | |||||
学位名 | ||||||
学位名 | 博士(歯学) | |||||
学位授与機関 | ||||||
学位授与機関識別子Scheme | kakenhi | |||||
学位授与機関識別子 | 34408 | |||||
学位授与機関名 | 大阪歯科大学 | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2014-03-07 | |||||
学位授与番号 | ||||||
学位授与番号 | 甲第719号 |