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Effect of Emdogain-derived Oligopeptides on Human Microvascular Endothelial Cells in vitro
https://osaka-dent.repo.nii.ac.jp/records/55
https://osaka-dent.repo.nii.ac.jp/records/5557755977-180a-43d7-b9ac-69e7aed7e040
名前 / ファイル | ライセンス | アクション |
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学位論文 (271.1 kB)
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論文内容要旨・審査結果要旨 (236.1 kB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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公開日 | 2016-08-01 | |||||
本文言語 | ||||||
言語 | eng | |||||
タイトル | ||||||
タイトル | Effect of Emdogain-derived Oligopeptides on Human Microvascular Endothelial Cells in vitro | |||||
著者 |
高橋, 宰達
× 高橋, 宰達× TAKAHASHI, Saitatsu |
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キーワード | ||||||
主題Scheme | Other | |||||
主題 | ペプチド | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | エナメル基質誘導体 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 内皮細胞 | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Peptides | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Enamel matrix derivative | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Endothelial cells | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
資源タイプ | doctoral thesis | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Purpose: Emdogain (EMD) is derived from the tooth germ of juvenile swine, and is commonly used for periodontal tissue regeneration, including the formation of alveolar bone, in the treatment of periodontitis. However, because it originates from pig tissue, some patients choose not to be treated with EMD. The active component of EMD is a peptide sequence that corresponds to one of the pro-proteins as amelogenin II. Angiogenesis is one of the most critical events in the wound healing process and in periodontal regeneration. As such, this peptide may function as an angiogenic factor to stimulate cell differentiation and tissue regeneration. Methods: We characterized the effects of 100 ng/ml of synthetic peptide by using matrix-assisted laser-desorption ionization time-of-flight mass spectrometry analysis of eosinophilic round bodies formed after subcutaneous injection of EMD into the backs of rats in a previous study on the proliferation after stimulation for 30 min or 1, 3, 6, 24 and 72 h, migration after stimulation for 1, 3 and 8 h, outgrowth extension after stimulation for 6 days, and ICAM-1 expression after stimulation for 3 days, in human microvascular endothelial cells (HMVECs). Results: We demonstrated that the EMD-derived peptide significantly increased HMVEC proliferation and chemotaxis over unstimulated controls after all stimulation times. The peptide also led to an increase in outgrowth of processes from HMVEC spheroids in three-dimensional collagen cultures. ICAM-1 mRNA expression was also significantly elevated in HMVECs following treatment with the EMD-derived peptide. Conclusion: EMD-derived synthetic peptide may act as an angiogenic factor to stimulate the proliferation, chemotaxis, adhesion and migration of microvascular endothelial cell. | |||||
学位名 | ||||||
学位名 | 博士(歯学) | |||||
学位授与機関 | ||||||
学位授与機関識別子Scheme | kakenhi | |||||
学位授与機関識別子 | 34408 | |||||
学位授与機関名 | 大阪歯科大学 | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2014-03-07 | |||||
学位授与番号 | ||||||
学位授与番号 | 甲第732号 |