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Human Gingival Integration-Free iPSCs; a Source for MSC-Like Cells
https://osaka-dent.repo.nii.ac.jp/records/103
https://osaka-dent.repo.nii.ac.jp/records/103b412e69b-8b4e-4dac-b4c9-d77d2436f7bc
名前 / ファイル | ライセンス | アクション |
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学位論文 (2.4 MB)
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論文内容要旨・審査結果要旨 (276.3 kB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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公開日 | 2016-08-01 | |||||
本文言語 | ||||||
言語 | eng | |||||
タイトル | ||||||
タイトル | Human Gingival Integration-Free iPSCs; a Source for MSC-Like Cells | |||||
著者 |
梅崎, 泰之
× 梅崎, 泰之× Umezaki, Yasuyuki |
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キーワード | ||||||
主題Scheme | Other | |||||
主題 | human gingival tissues | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | episomal vectors | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Mesenchymal | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | plasmid | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | pluripotent | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
資源タイプ | doctoral thesis | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Mesenchymal stem cells (MSCs) are considered a potential autologous therapy for tissue engineering. The available procedures for MSC retrieval from patients are invasive, and their limited in vitro proliferation restricts their use in the treatment of damaged tissues. Therefore, it is important to establish an alternative and safe source of MSCs. The objective of this study was to demonstrate induced pluripotent stem cell (iPSC) generation from a combination of an accessible source tissue and an integration-free method; we also attempted the differentiation of iPSCs into MSC-like cells (MSLCs) for future autologous tissue engineering. iPSCs were derived from human gingival tissues, which are easily accessible in the field of dentistry, via the use of non-integrating episomal plasmids. Established iPSCs expressed embryonic stem (ES) cell-specific markers, as assessed by gene analysis and immunocytochemistry. Embryoid bodies and teratoma formation were formed from iPSCs, showing their capacity to differentiate into three germ layers. Furthermore, we were successful in differentiating iPSCs into MSLCs. They tested positively for their capacity of trilineage differentiation. Our results demonstrate that human gingival integration-free iPSCs, readily accessible stem cells generated using episomal plasmid vectors, are a promising source of MSLCs, which can be used in tissue regeneration. | |||||
学位名 | ||||||
学位名 | 博士(歯学) | |||||
学位授与機関 | ||||||
学位授与機関識別子Scheme | kakenhi | |||||
学位授与機関識別子 | 34408 | |||||
学位授与機関名 | 大阪歯科大学 | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2016-03-11 | |||||
学位授与番号 | ||||||
学位授与番号 | 甲第768号 |