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Application of Green Tea Catechin for Inducing the Osteogenic Differentiation of Human Dedifferentiated Fat Cells in Vitro
https://osaka-dent.repo.nii.ac.jp/records/110
https://osaka-dent.repo.nii.ac.jp/records/110163f3245-6664-47fc-9cc8-af12af8e5747
名前 / ファイル | ライセンス | アクション |
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学位論文 (1.1 MB)
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論文内容要旨・審査結果要旨 (217.6 kB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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公開日 | 2016-08-01 | |||||
本文言語 | ||||||
言語 | eng | |||||
タイトル | ||||||
タイトル | Application of Green Tea Catechin for Inducing the Osteogenic Differentiation of Human Dedifferentiated Fat Cells in Vitro | |||||
著者 |
海田, 浩治
× 海田, 浩治× Kaida, Koji |
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キーワード | ||||||
主題Scheme | Other | |||||
主題 | Human Dedifferentiated Fat Cells | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Catechin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Osteogenic | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
資源タイプ | doctoral thesis | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Despite the advances in stem cell biology, there are few effective techniques to promote the osteogenic differentiation of human primary dedifferentiated fat (DFAT) cells. We attempted to investigate whether epigallocatechin-3-gallate (EGCG), which is the main component of green tea catechin, facilitates early osteogenic differentiation and mineralization on DFAT cells in vitro. DFAT cells were treated with EGCG (1.25–10 μM) in two osteogenic media with or without 100 nM dexamethasone [OM, OM(DEX)] for 12 days. Supplementation of 1.25 μM EGCG to both the media effectively increased the mRNA expression of collagen 1 (Col1A1) and runt-related transcription factor 2 (RUNX2), and also increased proliferation and mineralization. Compared with OM(DEX) with EGCG, OM with EGCG induced earlier COL1A1 and RUNX2 expression at day 1 and resulted in lower alkaline phosphatase staining at day 6, but higher mineralization at day 12. OM (DEX) with 10 μM EGCG remarkably hampered the proliferation of the DFAT cells. These results suggest that OM without DEX might be a preferable medium to promote proliferation and to induce osteoblast differentiation of DFAT cells. Our findings provide evidence for the potential use of EGCG and DFAT cells for bone regeneration and stem cell-based therapy. | |||||
学位名 | ||||||
学位名 | 博士(歯学) | |||||
学位授与機関 | ||||||
学位授与機関識別子Scheme | kakenhi | |||||
学位授与機関識別子 | 34408 | |||||
学位授与機関名 | 大阪歯科大学 | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2016-03-11 | |||||
学位授与番号 | ||||||
学位授与番号 | 甲第774号 |