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アイテム
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Accelerated generation of human induced pluripotent stem cells from human oral mucosa using episomal plasmid vectors and maternal transcription factor Glis1
https://osaka-dent.repo.nii.ac.jp/records/112
https://osaka-dent.repo.nii.ac.jp/records/11216244daf-35e8-477d-a666-e164e3daae1c
名前 / ファイル | ライセンス | アクション |
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学位論文 (533.1 kB)
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論文内容要旨・審査結果要旨 (222.3 kB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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公開日 | 2016-08-01 | |||||
本文言語 | ||||||
言語 | eng | |||||
タイトル | ||||||
タイトル | Accelerated generation of human induced pluripotent stem cells from human oral mucosa using episomal plasmid vectors and maternal transcription factor Glis1 | |||||
著者 |
柏木, 隆宏
× 柏木, 隆宏× Kashiwagi, Takahiro |
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キーワード | ||||||
主題Scheme | Other | |||||
主題 | iPSC | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | integration-free plasmid vector | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Glis1 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | human oral mucosal tissue | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
資源タイプ | doctoral thesis | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Objective: Induced pluripotent stem cells (iPSCs) possess high pluripotency and differentiation potential and may constitute a possible source of autologous stem cells for clinical applications. However, the lengthy reprogramming process (up to 1 month) remains one of the most significant challenges facing standard virus-mediated methodology. The transcription factor Gli-like transcription factor Glis1 is highly expressed in unfertilized eggs and one-cell-stage embryos. In this study, iPSCs were generated using a combination of primary human oral mucosa fibroblasts (HOFs) and episomal plasmid vectors expressing transcription factors including Glis1. Materials and Methods: HOFs were established from 3-mm in diameter oral mucosa tissue using the skin trephine of a 23-year-old Asian man. Human iPSCs were generated from the established HOFs using the following episomal plasmid vectors: pCXLE-hOCT3/4-shp53-F that expresses OCT3/4 and shRNA against p53; pCXLE-hSK that expresses SOX2 and KLF4; pCXLE-hUL that expresses L-MYC and LIN28; and pCXLE-hGlis1 that expresses Glis1. Results: Fifty colonies of human embryonic stem (ES)-like cells were observed as early as 20 days following initial episomal plasmid vector transduction. The resulting cell lines shared several characteristics with human ES cells, including morphology, pluripotency-associated gene and protein markers, karyotype analysis, and the ability to differentiate in vivo into all three germ layers. Conclusions: Our method, combining the use of HOFs and episomal plasmid vectors expressing OCT3/4, SOX2, KLF4, L-MYC, shRNA against p53, LIN28, and Glis1, offers a powerful tool for safely and rapidly generating bona fide human iPSCs and facilitates the application of iPSC technology to biomedical research. |
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学位名 | ||||||
学位名 | 博士(歯学) | |||||
学位授与機関 | ||||||
学位授与機関識別子Scheme | kakenhi | |||||
学位授与機関識別子 | 34408 | |||||
学位授与機関名 | 大阪歯科大学 | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2016-03-11 | |||||
学位授与番号 | ||||||
学位授与番号 | 甲第776号 |