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Effect of cyclic compressive loading on redifferentiation of human chondrocytes in three-dimensional cultured tissue
https://osaka-dent.repo.nii.ac.jp/records/150
https://osaka-dent.repo.nii.ac.jp/records/1508f83918d-a79c-4c9a-a797-9672c8e4f7aa
名前 / ファイル | ライセンス | アクション |
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学位論文 (443.1 kB)
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論文内容要旨・審査結果要旨 (356.5 kB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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公開日 | 2017-04-19 | |||||
本文言語 | ||||||
言語 | eng | |||||
タイトル | ||||||
タイトル | Effect of cyclic compressive loading on redifferentiation of human chondrocytes in three-dimensional cultured tissue | |||||
著者 |
岡本, 知子
× 岡本, 知子× Okamoto, Tomoko |
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キーワード | ||||||
主題Scheme | Other | |||||
主題 | Three-dimensional tissue | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Human chondrocytes | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Redifferentiation | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
資源タイプ | doctoral thesis | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Mechanical stress causes cartilage destruction in the temporomandibular joint (TMJ) and dedifferentiation of chondrocytes in TMJ cartilage. Although dedifferentiated chondrocytes redifferentiate in vitro under certain culture conditions with growth factors such as BMPs and TGF-β or mechanical loading, the effect of mechanical stress on redifferentiation of chondrocytes is unclear. We attempted to elucidate the effect of cyclic compressive loading on the redifferentiation of dedifferentiated human chondrocytes in three-dimensional (3D) culture. Our hypothesis was that cyclic compressive loads alter gene expression in an early stage of cartilage redifferentiation. Dedifferentiated human chondrocytes in monolayer were seeded onto a collagen scaffold to produce 3D tissue and were cultured with BMP-2 (500 ng/ml) and TGF-β3 (10 ng/ml) for 28 days. The effect of cyclic compressive loading of 20kPa at 0.5 Hz for 1 hour per day on the 3D tissue was analyzed for Sox9, BMP2, Aggrecan and type II collagen gene expressions on days 1, 4 and 7. Three-dimensional constructs with BMP-2 and TGF-β3 exhibited positive Safranin O staining on day 28. mRNA expression levels of Sox9 and Aggrecan gene were upregulated on day 4 with BMP-2 and TGF-β3, and type II collagen gene expression was also upregulated on day 7. Cyclic compressive loading on the 3D constructs further upregulated Sox9 and BMP2 gene expression on day 4, and BMP2 gene expression was also upregulated on day 7. We concluded that cyclic compressive loading alters gene expression during the early stage of cartilage redifferentiation. |
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学位名 | ||||||
学位名 | 博士(歯学) | |||||
学位授与機関 | ||||||
学位授与機関識別子Scheme | kakenhi | |||||
学位授与機関識別子 | 34408 | |||||
学位授与機関名 | 大阪歯科大学 | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2017-03-10 | |||||
学位授与番号 | ||||||
学位授与番号 | 甲第792号 |