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Study on Photodynamic Therapy with 8-MOP for Oral Mucosal Disease
https://osaka-dent.repo.nii.ac.jp/records/187
https://osaka-dent.repo.nii.ac.jp/records/1873b1241d4-9661-47a7-87c3-ce1bea48fc50
名前 / ファイル | ライセンス | アクション |
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学位論文 (973.8 kB)
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論文内容要旨・審査結果要旨 (211.4 kB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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公開日 | 2018-05-17 | |||||
本文言語 | ||||||
言語 | eng | |||||
タイトル | ||||||
タイトル | Study on Photodynamic Therapy with 8-MOP for Oral Mucosal Disease | |||||
著者 |
石川, 敬彬
× 石川, 敬彬 |
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キーワード | ||||||
主題Scheme | Other | |||||
主題 | Phototoxicity|8-MOP|3D culture | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_db06 | |||||
資源タイプ | doctoral thesis | |||||
アクセス権 | ||||||
アクセス権 | open access | |||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Psoralen ultraviolet A (PUVA) therapy using the cytotoxicity of ultraviolet radiation combined with 8-methoxypsoralen (8-MOP) is performed in the field of dermatol-ogy. As there are many disorders common to the oral mucosa and skin, treat-ments for refractory disorders of the skim may also be applicable to oral mucosal disorders. We, therefore, performed phototoxicity tests using 8-MOP primarily on cultured cells from oral tissues. The cell lines used were the mouse Balb/3T3, rat osteosarcoma UMR-106, rat squamous cell carcinoma SCC-158, human oral squamous cell carcinoma HSC-2, and human tongue squamous cell carcinoma HSC-3 and HSC-4. 8-MOP was added to the culture medium in 2-dimensional culture and 3-dimensional cul-ture using porcine Type I collagen gel. The cells were irradiated with ultraviolet light at 375 nm using an LED irradiation system. The control group was not irradiated. After 24-hour static culture, the cell survival rate was determined by MTT assay. In 2-dimensional culture, strong phototoxicity tended to be observed in cells de-rived from tumors. In 3-dimensional culture, phototoxicity varied in strength among cell types, and it was milder than in 2-dimensional culture, suggesting insufficient transmission of ultraviolet light to cells through the collagen gel. In cultured cells derived from tumors, the effects of photoirradiation varied among cell lines. As collagen may have inhibited transmission of ultraviolet light, a method to more ef-ficiently deliver ultraviolet light to cells in deep areas of the body is needed. |
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学位名 | ||||||
学位名 | 博士(歯学) | |||||
学位授与機関 | ||||||
学位授与機関識別子Scheme | kakenhi | |||||
学位授与機関識別子 | 34408 | |||||
学位授与機関名 | 大阪歯科大学 | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2018-03-09 | |||||
学位授与番号 | ||||||
学位授与番号 | 甲第825号 |