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乳清中のLPS/TLR 4シグナル抑制分子の検索
https://seitoku.repo.nii.ac.jp/records/839
https://seitoku.repo.nii.ac.jp/records/839bae506b4-0702-41f4-819c-632893c30b70
名前 / ファイル | ライセンス | アクション |
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201930_077-083 (529.0 kB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2020-05-18 | |||||
タイトル | ||||||
タイトル | 乳清中のLPS/TLR 4シグナル抑制分子の検索 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Research of LPS/TLR4 Signal Transduction Suppressor Molecules in Cow Whey | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 乳清 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Toll 様受容体4(TLR 4) | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 抗炎症性 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 破骨細胞 | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | whey | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Toll-like receptor 4(TLR 4) | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | anti-inflammatory | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | osteoclasts | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | departmental bulletin paper | |||||
著者 |
横山, 嘉子
× 横山, 嘉子× 藤原, しのぶ× 岩崎, 有希× 白石, 弘美× 加納, 和孝× Yoshiko, YOKOYAMA× Shinobu, FUJIHARA× Yuki, IWASAKI× Hiromi, SHIRAISHI× Kazutaka, KANO |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | 要旨 【目的】本研究では牛乳清中のLPS/TLR 4シグナル抑制分子の検索を目的とした。【方法】TLR 4/CD14/MD2-HEK293 細胞及びRAW264.7 細胞を用い、LPS/TLR 4シグナルに対する乳清の阻害効果を調べた。【結果】乳清溶液では、LPS/TLR 4シグナルを介した、TLR 4/CD14/MD2-HEK293 細胞でのSEAP 発現、及びRAW 264.7 細胞接着の阻害が確認された。さらに、Superdex 75 10/300 GL クロマトグラフィーにより、LPS 刺激で誘導されるTLR 4/CD14/MD2-HEK293 細胞のSEAP 発現、及びRAW264.7 細胞からのTRAP 陽性多核巨細胞の形成を抑制する、proteinase K 感受性の分子量約7,000 の画分が得られた。【結論】乳清中に、従来報告のない分子量約7,000のLPS/TLR 4シグナル抑制分子の存在を見出した。 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Abstract 【Objective】The purpose of this study was to research LPS/TLR4 signal suppressor molecules in cow whey. 【Procedure】TLR4/CD14/MD2-HEK293 and RAW264.7 cells were used to examine the inhibitory effect of whey on LPS/TLR4 signal transduction.【Results】In whey lysates, inhibition of SEAP expression in TLR4/ CD14/MD2-HEK293 cells via LPS /TLR4 signals, as well as inhibition of RAW264.7 cell adhesion via LPS/TLR4 signals was confirmed. Furthermore, Superdex 75 10/300 GL chromatography yields a proteinase K sensitive fraction with a molecular weight of approximately 7,000 that suppresses LPS stimulated expression of SEAP in TLR4/CD14/MD2-HEK293 cells and also suppresses LPS stimulated formation of TRAP-positive multinuclear giant cells from RAW264.7 cells.【Conclusion】In this study, we discovered the existence of LPS/TLR4 signal suppressor molecule of about 7,000 in cow whey. |
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書誌情報 |
研究紀要 en : Bulletin of Seitoku University, Bulletin of Seitoku University Junior College 巻 30, p. 77-83, 発行日 2019 |
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出版者 | ||||||
出版者 | 聖徳大学 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 21876843 | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | ||||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |