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バイオイメージングによる筋細胞内カルシウムイオン動態の評価
https://uec.repo.nii.ac.jp/records/1142
https://uec.repo.nii.ac.jp/records/11421a591fb6-1899-4c12-8670-93e850c45a4e
名前 / ファイル | ライセンス | アクション |
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9000000342.pdf (12.1 MB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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公開日 | 2009-03-24 | |||||
タイトル | ||||||
言語 | ja | |||||
タイトル | バイオイメージングによる筋細胞内カルシウムイオン動態の評価 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Evaluation of intracellular calcium ion in skeletal muscle using in vivo Bio-imaging | |||||
言語 | ||||||
言語 | jpn | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_46ec | |||||
資源タイプ | thesis | |||||
著者 |
曽野部, 崇
× 曽野部, 崇 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | The present study describes that to establish the methods of measurementintramyocyte Ca2+ level under in vivo condition. I used rat spinotrapezius muscle withfluorescence imaging technique, and succeeded to observe in vivo skeletal muscle Ca2+level. Because of the difficulty in measuring [Ca2+]i under in vivo conditions, almostall investigations of [Ca2+]i have been performed under in vitro conditions.Bioimaging techniques can visualize intracellular ions directly, and, whereasmeasurements of [Ca2+]i are potentially feasible under intravital conditions routinelyused for microcirculation studies, to date, because skeletal muscle was considered to betoo thick for microscopy, most studies of [Ca2+]i have used isolated or cultured singlemyocytes. Unfortunately, such isolated or cultured cells have quite a differentenvironment than in vivo skeletal muscle with respect to their absence of amicrocirculation, different oxygen and substrate availabilities, and metabolism, amongother considerations. The purpose of present investigation was to test the originalchallenges in the spinotrapezius muscle, and I demonstrated following results.1) Compared with the surgically excised in vitro spinotrapezius, the in vivo preparation(i.e., exteriorized, as for intravital miscroscopy) prolonged (90 min) observation at restdid not elevate [Ca2+]i. 2) An extended series of eccentric contractions elevated[Ca2+]i to a greater extent than isometric contractions. Moreover, the elevated [Ca2+]iaccompanying ECC contractions was prevented or substantially reduced by the SACblockers. 3) Females maintain [Ca2+]i homeostasis following novel eccentriccontractions whereas Males do not which is consistent with a role for elevated [Ca2+]i ineccentric exercise-induced muscle damage. 4) Diabetes muscles show vulnerability of[Ca2+]i homeostasis for increase in oxygen demand during isometric contraction. | |||||
学位名 | ||||||
学位名 | 博士(理学) | |||||
学位授与機関 | ||||||
学位授与機関名 | 電気通信大学 | |||||
学位授与年度 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 2008 | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2009-03-24 |