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Effect of hypoxia on progesterone production by cultured bovine early and mid luteal cells
https://obihiro.repo.nii.ac.jp/records/4337
https://obihiro.repo.nii.ac.jp/records/43375d941e73-9e7a-4e90-8463-865790aea511
名前 / ファイル | ライセンス | アクション |
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full text (714.7 kB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2019-03-14 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Effect of hypoxia on progesterone production by cultured bovine early and mid luteal cells | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Corpus luteum | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Early and mid luteal cells | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Hypoxia | |||||
キーワード | ||||||
言語 | en | |||||
主題Scheme | Other | |||||
主題 | Progesterone | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Hasegawa, Hiroki
× Hasegawa, Hiroki× Nishimura, Ryo× Yamashita, Masamichi× Yamaguchi, Takeshi× Hishinuma, Mitsugu× Okuda, Kiyoshi |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | A major role of the corpus luteum (CL) is to produce progesterone (P4). The CL has immature vasculature shortly after ovulation, suggesting it exists under hypoxic conditions. To elucidate the mechanism involved in regulation of luteal cell function during CL development, we compared the effect of hypoxia on P4 production by cultured bovine early and mid luteal cells. Luteal cells obtained from early and mid CL were incubated under different O2 concentrations (20% and 3%) with or without hCG (1 U/ml) for 6 h and 24 h. After 6 h of culture in the presence of hCG, P4 production was not affected by hypoxia whereas decrease in its production by mid luteal cells was observed. After 24 h of culture, P4 production was significantly decreased by hypoxia in both stages of luteal cells regardless of the use of hCG. At 6 h of culture, hypoxia increased mRNA expression of hydroxyl-Δ-5-steroid dehydrogenase, 3β- and steroid Δ-isomerase 1 (HSD3B1) in early luteal cells, and decreased mRNA expression of cytochrome P450 cholesterol side chain cleavage (CYP11A1) enzyme in mid luteal cells. At 24 h of culture, mRNA expressions of steroidogenic acute regulatory protein (STAR), CYP11A1, and HSD3B1 were not affected by hypoxia in both stages of luteal cells. The overall results suggest that early luteal cells maintain P4 production under hypoxic conditions, and hypoxia-induced HSD3B1 may support this P4 production in the bovine early CL. | |||||
言語 | en | |||||
書誌情報 |
en : The Journal of reproduction and development 巻 65, 号 1, p. 67-72, 発行日 2019-02 |
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出版者 | ||||||
言語 | en | |||||
出版者 | Society for Reproduction and Development | |||||
ISSN | ||||||
収録物識別子タイプ | PISSN | |||||
収録物識別子 | 13484400 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA12018734 | |||||
DOI | ||||||
関連タイプ | isIdenticalTo | |||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1262/jrd.2018-061 | |||||
権利 | ||||||
言語 | en | |||||
権利情報 | This is an open access article licensed under the terms of the Creative Commons Attribution (by) License. <http://creativecommons.org/lisenses/by/4.1/> | |||||
フォーマット | ||||||
内容記述タイプ | Other | |||||
内容記述 | application/pdf | |||||
著者版フラグ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
日本十進分類法 | ||||||
主題Scheme | NDC | |||||
主題 | 645 |