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Pam3CSK4, a TLR2 Agonist, Induces Osteoclastogenesis in RAW 264.7 Cells
https://showa.repo.nii.ac.jp/records/1032
https://showa.repo.nii.ac.jp/records/1032a43a61b5-2bb8-4b77-9bda-2289bf0c1ee8
名前 / ファイル | ライセンス | アクション |
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D32_181.pdf (975.6 kB)
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Item type | 学内発行雑誌 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2016-11-17 | |||||
タイトル | ||||||
タイトル | Pam3CSK4, a TLR2 Agonist, Induces Osteoclastogenesis in RAW 264.7 Cells | |||||
言語 | ||||||
言語 | eng | |||||
資源タイプ | ||||||
資源タイプ | departmental bulletin paper | |||||
著者 |
YANO, Akiko
× YANO, Akiko× SUZUKI, Keiko× YAMAMOTO, Matsuo× YAMADA, Shoji |
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書誌情報 |
Dental medicine research 巻 32, 号 3, p. 181-188, 発行日 2012-11 |
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抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | To clarify whether Pam3CSK4, a TLR2 agonist, induces the differentiation of osteoclasts, we investigated the osteoclastogenesis and gene expression induced by Pam3CSK4 in RAW264.7 monocyte/macrophage cells. We found that 1 μg/ml Pam3CSK4 induced osteoclastogenesis without adding RANKL exogenously, whereas 1 μg/ml LPS (Re mutant), a ligand for TLR4, failed to produce osteoclasts in RAW 264.7 cells. The number of TRAP-positive multinuclear cells in the Pam3CSK4 group (156.2 +/− 26.5 cells/well) was significantly (p<0.01) less than that of 100 ng/ml RANKL (196.5 +/− 32.0 cells/well), which was a positive control. Quantitative real-time RT-PCR analysis showed that i) the gene expression levels of TRAP, cathepsin K and matrix metalloproteinase 9, which are osteoclast differentiation markers, were upregulated (p<0.01) by both RANKL and Pam3CSK4, whereas LPS did not increase gene expression of TRAP or cathepsin K, ii) the expression level of RANK was decreased significantly (p<0.01) by both Pam3CSK4 and LPS, but increased by RANKL, iii) the expression levels of TNFα and IL-6, inflammatory cytokines, were upregulated significantly (p<0.01) by both Pam3CSK4 and LPS and iv) the expression level of RANKL was similar to that of other experimental groups in RAW 264.7 cells (p>0.05). Collectively, these results indicate that Pam3CSK4, but not LPS, induces osteoclastogenesis in RAW 264.7 cells in the absence of exogenous RANKL. | |||||
DOI | ||||||
関連識別子 | 10.7881/dentalmedres.32.181 | |||||
出版者 | ||||||
出版者 | 昭和大学・昭和歯学会 | |||||
ISSN | ||||||
収録物識別子 | 1882-0719 | |||||
著者版フラグ | ||||||
出版タイプ | VoR |